Stem Cell Culture

Contributors to Volume 86 Preface Biography Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions I Introduction II Methods III Discussion IV Materials Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and Feeder‐Free Conditions I Introduction II Methods and Materials III Results IV Discussion and Summary Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation I Introduction II Rationale III Methods IV Summary Culture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal Animal I Introduction II Rationale III Methods IV Materials V Discussion Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability I Introduction II Amniotic Fluid Cell Composition and Stem Cells III In Vitro Potential of Amniotic Fluid Stem Cells IV Ex Vivo and In Vivo Application of Amniotic Fluid Stem Cells V Discussion Method to Isolate Mesenchymal‐Like Cells from Wharton's Jelly of Umbilical Cord I Introduction II Rationale III Methods IV Materials V UCMSCs and Culture Characteristics VI Discussion Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs) I Introduction II Methods III Concluding Remarks Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance I Introduction II Epithelial–Mesenchymal Relationship III Stem Cells and Lineage Biology IV Liver Processing V Fractionation of Liver Cell Subpopulations VI Feeder Cells VII Extracellular Matrix VIII Culture Media IX Monolayer Cultures of Cells X Three‐Dimensional Systems Acknowledgments Appendix 1: Nomenclature, Glossary, and Abbreviations Appendix 2: Details on the Preparation of Buffers/Reagents Appendix 3: Commercial Sources of Reagents Appendix 4: Sources of Primary and Secondary Antibodies for Immunoselection and for Characterization of the Cells. (See Below for Suppliers and the Location of the Suppliers) Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo I Introduction II Rationale III Methods IV Materials V Discussion VI Summary Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells I Introduction II Rationale III Methods IV Materials V Discussion VI Summary Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue I Introduction II Isolation of hMSCs from Trabecular Bone III Isolation of hMSCs from Adipose Tissue IV Growth, Subculture, and Cryopreservation of Undifferentiated hMSCs V Surface Marker Characterization of hMSCs VI hMSC Differentiation Assays for Osteogenic, Adipogenic, and Chondrogenic Pathways VII Summary Culture of Mesenchymal Stem/Progenitor Cells in Adhesion‐Independent Conditions I Introduction II Suspension Culture of Bone Marrow‐Derived MPCs III Analysis of Suspension Culture IV Mechanism of MPC Expansion in Suspension V Discussion Purification and Long‐Term Culture of Multipotent Progenitor Cells Affiliated with the Walls of Human Blood Vessels: Myoendothelial Cells and Pericytes I Introduction II Materials and Methods: Cell Analysis and Purification by Flow Cytometry III Materials and Methods: Long‐Term Culture of Human Blood Vessel‐Associated Progenitor Cells IV Materials and Methods: Proliferation Kinetics of Human Myoendothelial Cells and Pericytes V Materials and Methods: Genotypic and Phenotypic Analyses of Long‐Term Cultured Cells VI Conclusion Isolation and Culture of Colon Cancer Stem Cells I Introduction II Identification of CSCs in Colon Cancer Tissue III Isolation and Propagation of Colon CSCs IV Discussion Isolation and Establishment of Human Tumor Stem Cells I Introduction II Rationale III Methods IV Results V Discussion VI Materials Stem Cells from Cartilaginous and Bony Fish I Introduction to Models and Uses II Embryonal Stem Cells III Tissue‐Specific Stem Cells IV Outlook and Future Contributions Index Volume in Series