Total Internal Reflection Fluorescence (TIRF) and Evanescence Microscopies

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Daniel Axelrod (Professor Emeritus, Physics and Biophysics, University of Michigan (United States))

Total Internal Reflection Fluorescence

TIRF and Evanescence Microscopies

Daniel Axelrod (Professor Emeritus, Physics and Biophysics, University of Michigan (United States))

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English

Institute of Physics Publishing
13 September 2022

Microscopy; Biophysics; Applied optics

Series: Biophysical Society-IOP Series

This book offers a complete presentation of the physics, math, and experimental setups for both TIRF and related evanescence microscopies. It covers evanescence in both fluorescence excitation or emission. It also discusses, in detail, the theory, setups, and practical biological/biochemical applications for combinations of evanescence microscopies with other optical techniques such as polarization, photobleaching, correlation spectroscopy, scattering, image enhancement, optical force, two-photon, energy transfer, structured illumination, scanning, quenching, and single molecule detection. Physical and qualitative discussions augment the rigorous math, making the book accessible and interesting to a wide range of audiences with backgrounds from biology to chemistry to physics. The book also contains useful step-by-step guides to building, modifying, and aligning TIRF microscopy systems for specialized purposes.

Key Features:

Suitable for both basic and advanced levels

Offers combinations with related techniques

Relationship of evanescent effects between excitation and emission explained

Practical guide to the optics as well

By: Daniel Axelrod (Professor Emeritus Physics and Biophysics University of Michigan (United States))
Imprint: Institute of Physics Publishing
Country of Publication: United Kingdom
Dimensions: Height: 254mm, Width: 178mm, Spine: 13mm
ISBN: 9780750333498
ISBN 10: 0750333499
Series: Biophysical Society-IOP Series
Pages: 197
Publication Date: 13 September 2022
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active

CHAPTER I. Introduction to Optical Evanescence CHAPTER II : Total internal reflection theory CHAPTER III: Structure in the lower index material CHAPTER IV: Emission of fluorophores near a surface CHAPTER IV: Emission of fluorophores near a surface CHAPTER V: Optical configurations and setup CHAPTER VI: Applications and combinations with other techniques

Daniel Axelrod is a Professor Emeritus of Physics and Biophysics at the University of Michigan, Ann Arbor. His specialty is developing novel optical microscopy techniques to study the motion and organization of biological molecules and cellular organelles near biological surfaces. Many biological aspects are done in collaboration with research groups at the University of Michigan Medical School. Professor Axelrod is a Fellow of the Biophysical Society and a recipient of the Biophysical Society’s 2010 Gregorio Weber Award for fluorescence theory and applications.