DNA Damage and Chromosomal Instability

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Christian Zierhut Lorenzo Galluzzi (Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA)

DNA Damage and Chromosomal Instability

Christian Zierhut, Lorenzo Galluzzi (Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA)

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English

Academic Press Inc
12 February 2024

Developmental biology; Molecular biology

Series: Methods in Cell Biology

DNA Damage and Chromosomal Instability, Volume 182 in the Methods in Cell Biology series, presents interesting chapters on a variety of timely topics, including Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation, Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents, Genetic reporters to detect and quantify homologous recombination in yeast, Mapping histone variant genomic distribution: exploiting SNAP- Tag labelling to follow the dynamics of incorporation of H3 variants, Enrichment of DNA replication intermediates by EdU pull down, Nascent DNA sequencing and its diverse applications in genome integrity research, and much more. Additional chapters cover Enrichment of DNA replication intermediates by EdU pull down, DNA curtains for studying phase separation mechanisms of DNA-organizing proteins, Visualizing the Dynamics of DNA Replication and Repair at the Single-Molecule Molecule Level, Cloning and Expansion of Repetitive DNA Sequences, Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy Arne Lindqvist

Volume editor: Christian Zierhut, Lorenzo Galluzzi (Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA)
Imprint: Academic Press Inc
Country of Publication: United States
Dimensions: Height: 235mm, Width: 191mm,
Weight: 450g
ISBN: 9780443188985
ISBN 10: 044318898X
Series: Methods in Cell Biology
Pages: 318
Publication Date: 12 February 2024
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Reviews for DNA Damage and Chromosomal Instability

""...addresses experimental methods for inducing DNA damage and chromosome instability, methods for evaluating the mechanisms of causation, and approaches to the study of the consequences of these events. It also explores mechanisms of repair of DNA and chromosome damage,... the cumulative work of 55 authors.... [H]ighly technically focused,... each chapter begins with a short abstract followed by introductory and background information. Detailed laboratory methods follow the introduction, and these include step-by-step instructions for the procedures discussed.... [U]niquely easy to use for active researchers . Overall, this volume accomplishes what it sets out to do quite well. The detailed laboratory methods presented in each chapter are a strong point of the book."" --©Doody’s Review Service, 2024, Mark F. Sanders, PhD (UC Davis College of Biological Sciences)