Western Blotting for the Non-Expert

Western blotting: How it began.- 2. Basics of Western Blotting.- 3.Basic Calculations in Western Blotting.- 4.Choosing The Right Antibody For Western Blotting.- 5. Sample Preparation.- 6. Blocking membranes at places that do not have transferred proteins.- 7. A Basic Method for Western Blotting.- 8. Trouble Shooting Western Blotting.- 9. Using Biotin to demonstrate western and dot blotting in University Practical Classes- A Guide To Instructors.- 10. Nitrocellulose Membrane Art.- 11. The Use Of A Harmless Invisible Ink To Send Secret Messages On Nitrocellulose Membranes And As A Substitute For The Harmful Radioactive Ink Used As A Marker In ECL Membrane Assays.- 12. Fingerprinting On Nitrocellulose And Polyvinylidene Difluoride Membrane.- 13. Spectrometric Determination Of Protein For Electrophoresis.- 14. Concentrating Proteins For Western Blotting.- 15. Dissolving Proteins Using Lysis Buffer.- 16. Blotting AndDetecting Glycosaminoglycans.- 17. Passive Blotting From Tissue Slices.- 18. Localizing Proteins by Tissue Printing.- 19. Ultrasound Blotting.- 20. Obtaining Several Blots From A Single SDS-PAGE Gel By Passive Transfer.- 21. Diffusion Blotting From SDS-PAGE Gels Supported By Solid Plastic.- 22. Dot-Immunobinding.- 23. Simultaneous Immunoblotting Analysis With Activity Gel Electrophoresis.- 24. Protein Blotting By Centrifugation.- 25. Blotting And Immunostaining Of Multiple Antigenic Peptides.- 26. High Molecular Weight Protein Blotting Using Agarose Gel Electrophoresis.- 27. Cetyltrimethylammonium bromide PAGE And Eastern Blotting.- 28. Purification Of Autoantibodies Bound To An Autoantigen Immobilized On A Membrane Strip.- 29. Miniaturized Blotting.- 30. Affinity Immunoblotting.- 31. Transferring Coomassie Blue Stained Proteins from Polyacrylamide Gels To Transparencies.- 32. Protein Transfer From Immobilized pH Gradient Gels.- 33. SDS PAGE To Immunoblotting In Sixty Minutes.- 34. Western Blot Analysis Of Protein-DNA Complexes Formed DuringGel Shift Experiments.- 35. Electrophoretic Transfer Of High And Low Molecular Weight Proteins To Membranes Using Heat.- 36. Grid-immunoblotting.- 37. Far-western Blotting.- 38. Native Electrophoresis And Western Blot Analysis.- 39.  Efficient Electroblotting Of Low Molecular Weight Protein After Staining With Coomassie.- 40. Avoiding Non-specific Binding Of Secondary Antibodies In Immunoblotting By Double-blotting.- 41.A Brief Introduction To Other Protein Blotting Methods.- 42. Quantitative Computerized Western Blotting In Detail.- 43. Common Problems In Electrophoresis.