Techniques in Light Microscopy

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Steven E. Ruzin (Emeritus Professor, Emeritus Professor, University of California, Berkeley)

Techniques in Light Microscopy

Steven E. Ruzin (Emeritus Professor, Emeritus Professor, University of California, Berkeley)

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English

Oxford University Press
12 December 2024

Microscopy; History of science; Light (optics)

This textbook provides an accessible and pedagogical explanation of the way microscopes magnify images and covers all techniques to date in transmitted and fluorescent light microscopy.

The first section covers basic optics as it relates to microscopy. The second section describes all the major optical techniques of transmitted light microscopy, starting with brightfield, through darkfield, polarized light, phase contrast, differential interference contrast, and Hoffman modulation contrast microscopy. The final third of the book covers all the techniques of fluorescence microscopy. It begins with a simple explanation of fluorescence and is followed by Widefield epifluorescence, confocal, and 2-photon microscopy. This is followed by computational imaging including restoration ('deconvolution') microscopy, and all the present super-resolution techniques. The book concludes by discussing attainable resolution using transmitted light microscopy, including a thorough discussion of the Rayleigh, Sparrow, and other criteria of resolution, ending with a short discussion of the common pitfalls that result in decreased microscope resolution. The final chapter in the book is a short history of the microscope, beginning with the ancients, then discussing three seminal natural philosophers: Leeuwenhoek, Swammerdam, and Hooke. The remaining sections of the history chapter cover mechanical and optical advancements in the history of transmitted light microscopy. Finally, it includes a short history of fluorescence microscopy starting with Köhler and Rohr's first use of fluorescence microscopy in 1905, and ending with a description of the Sarastro Phoibos 1000, the first commercial confocal microscope.

By: Steven E. Ruzin (Emeritus Professor Emeritus Professor University of California Berkeley)
Imprint: Oxford University Press
Country of Publication: United Kingdom
Dimensions: Height: 254mm, Width: 178mm, Spine: 19mm
Weight: 668g
ISBN: 9780198885832
ISBN 10: 0198885830
Pages: 256
Publication Date: 12 December 2024
Audience: College/higher education , Primary
Format: Hardback
Publisher's Status: Active

Part I 1: Light Microscope Optics 2: Lens Aberrations 3: The Compound Microscope Part II 4: Transmitted Light Techniques 5: Polarized Light Microscopy 6: Phase Contrast Microscopy 7: Differential Interference Contrast Microscopy Part III 8: Epifluorescence Microscopy 9: Selective/Single Plane Imaging Microscopy 10: Confocal Microscopy 11: Two-Photon Microscopy 12: Restoration Microscopy 13: Super-Resolution Microscopy 14: Resolution 15: A Brief History of the Light Microscope Appendix I: Digital imaging and sampling Appendix II: Synopsis of transmitted light microscopy techniques Appendix III: Synopsis of epifluorescence microscopy techniques

Steven E. Ruzin was Director of the CNR Biological Imaging Facility at the University of California, Berkeley from 1989 to 2022 and has been Curator of the Golub Collection of antique microscopes since 2004. He is now Emeritus Professor at the University of California, Berkley.

Reviews for Techniques in Light Microscopy

Very well written, clearly describing both traditional, widely-used microscopes and more recent, cutting-edge technologies. * Annette Chan, San Francisco State University * A splendid work that not only demonstrates the author's proficiency but also his ability to teach. * Christopher Guerin, University of Ghent, Belgium *