Single-Molecule Science

'Single-molecule experiments have revolutionized our approaches to biophysics and biochemistry at molecular scales. Only a handful of reference textbooks serves the ever-expanding community of practitioners, beginners, and curious amateurs of single-molecule techniques. This volume is poised to become a new single-molecule reference work, to be readily at hand in any bioscience laboratory, to be read and understood by any scientist practicing, or simply interested, in the new world of experiments, reasoning, and statistical analysis opened up by single-molecule science.' Michel A. G. J. Orrit, Professor of Spectroscopy of Molecules in Condensed Matter, Leiden University, The Netherlands 'An excellent collection of cutting-edge reports related to single-molecule spectrometry and imaging that consists of widespread contents from basic to application; both students [and] experts will find an extended application of the technologies to biological and clinical research.' Akira Watanabe, Assistant Professor of Life Science Frontiers, Kyoto University, Japan 'Gives an overview of the applications of single-molecule techniques in the biological sciences. It addresses topics as diverse as super-resolution microscopy (far beyond the Abbe limit) and the study of protein mechanics without ensemble averaging, and, due to its compact size, is informative reading for students and experts alike.' Lothar Kador, Professor of Physics, University of Bayreuth, Germany 'You will learn about the birth of detection of individual biomolecules, associated state-of-the-art technologies, cutting-edge research, and potential medical applications, all from a single-molecule point of view that will inform your new biological thoughts and insights.' Katsuyuki Shiroguchi, Principal Investigator, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan