Rational Design of Enzyme-Nanomaterials, the new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in rational design of enzyme-nanomaterials, and includes sections on such topics as conjugation of enzymes and dextran-aldehyde polymers, improved activity of enzymes bound to titanate nanosheet, nano-layered 'stable-on-the-table' biocatalysts and nanoparticle-based enzyme sensors.
1. Preparation of Biocatalytic Microparticles by Interfacial Self-Assembly of Enzyme-Nanoparticle Conjugates Around a Cross-Linkable Core Stephanie M. Andler, Li-Sheng Wang, Julie M. Goddard and Vincent M. Rotello 2. Monitoring Enzymatic Proteolysis Using Either Enzyme- or Substrate Bioconjugated Quantum Dots Sebastián A. Díaz , Joyce C. Breger and Igor L. Medintz 3. Intense Pegilation of enzyme surfaces: Relevant Stabilizing Effects Sonia Moreno-Pérez, Alejandro H. Orrego, María Romero, Lara Trobo-Maseda, Sandro Martins-DeOliveira, Roberto Munilla, Gloria Fernández-Lorente and Jose M. Guisan 4. Immobilization of Lipases on Heterofunctional Octyl-glyoxyl Agarose Supports: Improved Stability and Prevention of the Enzyme Desorption Nazzoly Rueda, Jose C. S. dos Santos, Rodrigo Torres, Claudia Ortiz, Oveimar Barbosa and Roberto Fernandez-Lafuente 5. Biomimetic/Bioinspired Design of Enzyme@capsule Nano/Micro-Systems Jiafu Shi, Yanjun Jiang, Shaohua Zhang, Dong Yang and Zhongyi Jiang 6. Synergistic Functions of Enzymes Bound to Semiconducting Layers Kai Kamada, Misato Kamiuchi, Motoko Tokunaga, Daiki Ito and Nobuaki Soh 7. Bioconjugation of Antibodies and Enzyme Labels onto Magnetic Beads Brunah A. Otieno, Colleen E. Krause and James F. Rusling 8. Rationally Designed, 'Stable-on-the-Table' nanoBiocatalysts bound to Zr(IV) Phosphate Nanosheets Inoka K. Deshapriya and Challa V. Kumar 9. Portable Enzyme-paper Biosensors Based on Redox Active CeO2 Nanoparticles Anahita Karimi, Ali Othman and Silvana Andreescu 10. Rational Design of Nanoparticle Platforms for ‘Cutting-the-Fat’: Covalent Immobilization of Lipase, Glycerol Kinase and Glycerol-3-Phosphate Oxidase on Metal Nanoparticles Vishakha Aggarwal and Chandra Shekhar Pundir 11. Biographene: Direct Exfoliation of Graphite in A Kitchen Blender for Enzymology Applications Challa Vijaya Kumar and Ajith Pattammattel
Department of Chemistry, University of Connecticut, USA
