Preparative Layer Chromatography

Teresa Kowalska, Joseph Sherma, Jack Cazes (Consultant, Lady Lake, Florida, USA) , Bernd Spangenberg (Fachhochschule Offenburg, Germany)

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English

CRC Press
30 June 2020

Chromatography; Biotechnology

Series: Chromatographic Science Series

Preparative Layer Chromatography explains how this method is used for separating large quantities of mixtures containing a wide variety of important compounds. It offers a broad review of preparative layer chromatography (PLC) applications and adaptable working procedures for microseparations involving organic, inorganic, and organometallic compounds.

The book contains theoretical background, chemical principles, and relevance of preparative layer chromatography (PLC) to a wide range of applications, particularly in the study of pharmaceuticals and biochemistry. Written by many of the best known and most knowledgeable specialists in the field, the chapters describe all the necessary techniques, current procedures, and superior strategies for selecting the most suitable eluents and designing application-specific PLC systems based on the data being sought. They provide comprehensive instructions, surrounding issues, and suggestions for optimizing optional working techniques within the framework of PLC. The book also provides a complete coverage of bulk sorbents and precoated chromatographic plates available on the international market.

A comprehensive, yet accessible source of information, Preparative Layer Chromatography is a relevant and practical text for experienced as well as novice researchers and practitioners involved in analytical, environmental, geochemical, biological, medicinal, and pharmaceutical analysis.

Contributions by: Bernd Spangenberg (Fachhochschule Offenburg Germany)
Edited by: Teresa Kowalska, Joseph Sherma, Joseph Sherma (Lafayette College, Easton, Pennsylvania, USA)
Series edited by: Jack Cazes (Consultant Lady Lake Florida USA)
Imprint: CRC Press
Country of Publication: United Kingdom
Dimensions: Height: 234mm, Width: 156mm,
Weight: 453g
ISBN: 9780367577919
ISBN 10: 0367577917
Series: Chromatographic Science Series
Pages: 424
Publication Date: 30 June 2020
Audience: College/higher education , Primary , Further / Higher Education
Format: Paperback
Publisher's Status: Active

Introduction. Adsorption Planar Chromatography in the Non-linear Range: Selected Drawbacks and Selected Guidelines. Sorbents and Precoated Layers in PLC. Selection and Optimization of the Mobile Phase for PLC. Sample Application and Development of Layers. On Methodical Possibilities of the Horizontal Chambers in PLC. Location of Separated Zones by Use of Visualization Reagents, UV Absorbance on Layers Containing a Fluorescent Indicator, and Densitometry. Additional Detection Methods for Separated Zones and Recovery of Substances from the Layer. Medical Applications of PLC. PLC of Hydrophobic Vitamins. PLC of Natural Mixtures. Application of PLC to Lipids. The Use of PLC for Separation of Natural Pigments. Application of PLC to Inorganics and Organometallics. PLC in a Clean-up and Group Fractionaction of Geochemical Samples: A Review of the Commonly Applied Techniques. The Use of PLC for Isolation and Identification of Unknown Compounds from the Frankincense Resin: Strategies for Finding Marker Substances.

Teresa Kowalska, Joseph Sherma

Reviews for Preparative Layer Chromatography

""...A valuable and important supplement to the existing vast chromatographic literature, [this book] demonstrates the potential of planar chromatography for separation and isolation of pure compounds, even from very complex mixtures."" -Journal of Liquid Chromatography, August 2006 ""The contributor[s']...experience integrated in the monograph forms a good basis for the readers."" -Instrumentation Science & Technology, August 2006 ""The [first] eight chapters provide sound theoretical and methodological foundations of PLC. Numerous applications of PLC are reviewed in the second section, based on extensive literature. ...The application reviews provide numerous examples of solving experimental difficulties for the separation of complex mixtures."" -Preparative Biochemistry & Biotechnology, August 2006 ""The book is quite well produced and has a large number of illustrations . . . this volume is a worthy addition to the PLC literature, and a good place to start for the novice."" – I.D. Wilson, in Chromatographia, January 2008, Vol. 67, No. 1/2