Discover the latest edition of this authoritative textbook on plant biotechnology and genetics
Plant biotechnology is a field of research and development in which scientific techniques are brought to bear on the creation and modification of new, beneficial plants and strains. Biotechnological techniques can be used to add nutritive value, increase resistance to diseases and pests, increase yields, and more. The production of biotech crops has increased over one hundred times since their introduction into commercial agriculture in 1996, making them the most rapidly-adopted crop category in the history of modern agriculture.
Plant Biotechnology and Genetics is the essential introduction to this thriving research subject. Beginning with an overview of basic plant biology and genetics, it then moves to the fundamental elements of biotechnology. Now fully updated to reflect the latest research advances and technological breakthroughs, it continues to be a must-own for readers interested in the future of food production and more.
Readers of the third edition of Plant Biotechnology and Genetics will also find:
New chapters covering topics like genome editing, chloroplast genome engineering, and synthetic biology Updates throughout to incorporate increased coverage of haploid production, genomic selection, and more Summary and discussion questions in each chapter, along with a companion website incorporating images and lecture materials
Plant Biotechnology and Genetics is ideal for advanced undergraduate and masters students in plant biotechnology courses, as well as professionals seeking a helpful reference guide.
Edited by:
C. Neal Stewart Jr. (University of Tennessee Knoxville TN)
Imprint: John Wiley & Sons Inc
Country of Publication: United States
Edition: 3rd edition
Dimensions:
Height: 257mm,
Width: 185mm,
Spine: 33mm
Weight: 907g
ISBN: 9781394217212
ISBN 10: 1394217218
Pages: 512
Publication Date: 27 March 2025
Audience:
Professional and scholarly
,
Undergraduate
Format: Hardback
Publisher's Status: Active
List of Contributors xv Preface xvii 1. The Impact of Biotechnology on Plant Agriculture 1 Graham Brookes 1.0. Chapter Summary and Objectives 1 1.1. Introduction 1 1.2. Cultivation of Biotechnology (GM) Crops 2 1.3. Why Farmers Use Biotech Crops 4 1.4. GM Crop Trait Use on Production and Farming 6 1.5. How the Adoption of Plant Biotechnology has Impacted the Environment 7 1.6. Conclusions 13 References 15 2. Mendelian Genetics and Plant Reproduction 17 Matthew D. Halfhill and Suzanne I. Warwick 2.0. Chapter Summary and Objectives 17 2.1. Genetics Overview 17 2.2. Mendelian Genetics 20 2.3. Mitosis and Meiosis 24 2.4. Plant Reproductive Biology 28 2.5. Conclusion 34 Life Box 2.1. Richard A. Dixon 35 Life Box 2.2. Michael L. Arnold 36 References 38 3. Plant Breeding 39 Nicholas A. Tinker and Elroy R. Cober 3.0. Chapter Summary and Objectives 39 3.1. Introduction 40 3.2. Central Concepts in Plant Breeding 41 3.3. Objectives in Plant Breeding 52 3.4. Methods of Plant Breeding 53 3.5. Breeding Enhancements 64 3.6. Conclusions 71 Life Box 3.1. Gurdev Singh Khush 71 Life Box 3.2. P. Stephen Baenziger 73 References 75 4. Plant Development and Physiology 76 Glenda E. Gillaspy and Catherine P. Freed 4.0. Chapter Summary and Objectives 76 4.1. Plant Anatomy and Morphology 77 4.2. Embryogenesis and Seed Germination 78 4.3. Meristems 84 4.4. Leaf Development 87 4.5. Flower Development 90 4.6. Hormone Physiology and Signal Transduction 93 4.7. Conclusions 99 Life Box 4.1. Natasha Raikhel 99 Life Box 4.2. Brenda S.J. Winkel 101 References 103 5. Tissue Culture: The Manipulation of Plant Development 105 Vinitha Cardoza 5.0. Chapter Summary and Objectives 105 5.1. Introduction 105 5.2. History of Tissue Culture 106 5.3. Media and Culture Conditions 107 5.4. Sterile Technique 109 5.5. Culture Conditions and Vessels 111 5.6. Culture Types and Their Uses 113 5.7. Regeneration Methods of Plants in Culture 119 5.8. Rooting of Shoots 122 5.9. Acclimation 122 5.10. Automation in Plant Tissue Culture 123 5.11. Artificial Intelligence (AI) and Machine Learning in Plant Tissue Culture 123 5.12. Problems That Can Occur in Tissue Culture 123 5.13. Conclusions 124 Acknowledgments 124 Life Box 5.1. Vinitha Cardoza 125 Life Box 5.2. Raymond D. Shillito 126 References 129 6. Molecular Genetics of Gene Expression 133 Maria Gallo and Alison K. Flynn 6.0. Chapter Summary and Objectives 133 6.1. The Gene 134 6.2. DNA Packaging into Eukaryotic Chromosomes 134 6.3. Transcription 135 6.4. Translation 144 6.5. Protein Postranslational Modification 150 Life Box 6.1. Maarten Chrispeels 150 Life Box 6.2. Hong S. Moon 152 References 153 7. Plant Systems Biology 155 Wusheng Liu, Yongil Yang, and C. Neal Stewart, Jr. 7.0. Chapter Summary and Objectives 155 7.1. Introduction 156 7.2. Defining Plant Systems Biology 157 7.3. Properties of Plant Systems 158 7.4. A Framework of Plant Systems Biology 160 7.5. Disciplines and Enabling tools of Plant Systems Biology 162 7.6. Conclusions 179 Life Box 7.1. C. Robin Buell 180 Life Box 7.2. Joshua Yuan 182 References 183 8. Recombinant DNA, Vector Design, and Construction 185 Stephen L. Gasior, David G.J. Mann, and Mark D. Curtis 8.0. Chapter Summary and Objectives 185 8.1. Plasmids are Unique Genetic Elements in Nature 186 8.2. DNA Vectors 189 8.3. Recombinant DNA Methods 195 8.4. Vector Design in Plant Research and Trait Development 206 8.5. Vectors for Targeted Genome Manipulations 213 8.6. Prospects 216 Life Box 8.1. David Mann 216 References 218 9. Genes and Traits of Interest 224 Joanna H. Kud and Kenneth L. Korth 9.0. Chapter Summary and Objectives 224 9.1. Introduction 225 9.2. Identifying Genes of Interest Via Omics Technologies 225 9.3. Traits for Improved Crop Production Using Transgenics 228 9.4. Conclusion 245 Life Box 9.1. Tony Shelton 246 References 247 10. Promoters and Marker Genes 249 Wusheng Liu, Debao Huang, C. Neal Stewart, Jr., and Brian Miki 10.0. Chapter Summary and Objectives 249 10.1. Introduction 250 10.2. Promoters 250 10.3. Marker Genes 259 10.4. Marker- Free Strategies 270 10.5. Conclusions 272 Life Box 10.1. Wusheng Liu 274 Life Box 10.2. Yunde Zhao 275 References 277 11. Transgenic Plant Production 282 John J. Finer and Ning Zhang 11.0. Chapter Summary and Objectives 282 11.1. Overview of Plant Transformation 283 11.2. Agrobacterium Tumefaciens 286 11.3. Particle Bombardment 293 11.4. Other Methods of Transformation 297 11.5. The Rush to Publish 300 11.6. A Look to the Future 306 Life Box 11.1. John Finer 306 Life Box 11.2. Kan Wang 308 Life Box 11.3. Ted Klein 310 References 312 12. Analysis of Transgenic Plants 315 C. Neal Stewart, Jr. 12.0. Chapter Summary and Objectives 315 12.1. Essential Elements of Transgenic Plant Analysis 316 12.2. Assays for Transgenicity, Insert Copy Number, and Segregation 317 12.3. Transgene Expression 323 12.4. Knockdown or Knockout Analysis Rather Than Overexpression Analysis 326 12.5. The Relationship Between Molecular Analyses and Phenotype 327 Life Box 12.1. Neal Stewart 327 Life Box 12.2. Nancy A. Reichert 329 References 331 13. Plastid Genetic Engineering 332 Alessandro Occhialini and Scott C. Lenaghan 13.0. Chapter Summary and Objectives 332 13.1. Introduction 333 13.2. Plastid Biology and Molecular Genetics 334 13.3. Plastid Genetic Engineering History and Motivations 336 13.4. Plastome Engineering Versus Nuclear Genome Engineering 336 13.5. Key Components for Plastome Engineering of Plants 338 13.6. Plastome Transformation Vector Design 340 13.7. Beyond Transplastomics: The Use of Episomal Vectors for Minisynplastome and Minichromosome Approaches 347 13.8. Removing DNA from Plastids 349 13.9. The Future of Plastid Engineering 350 Life Box 13.1. Henry Daniell 351 Life Box 13.2. Pal Maliga 354 Life Box 13.3. Alessandro Occhialini 356 Life Box 13.4. Ralph Bock 357 References 358 14. CRISPR- Cas: Genome Editing from Small- Scale to High Throughput for Plant Biology and Biotechnology 366 S.P. Avinash, Mirza J. Baig, and Kutubuddin A. Molla 14.0. Chapter Summary and Objectives 366 14.1. Introduction 367 14.2. Diverse CRISPR Approaches and Tools for Precisely Editing Genomes 371 14.3. Changing Gene Expression by CRISPR 374 14.4. CRISPR Screening for Large- Scale Functional Genomics 376 14.5. CRISPR- Enabled Crop Improvement 377 14.6. Commercialized Genome- Edited Crops 380 14.7. Conclusions 380 Life Box 14.1. Kutubuddin Molla 381 Life Box 14.2. Dan Voytas 382 Life Box 14.3. Yiping Qi 383 References 385 15. Regulations and Biosafety 390 Alan Mchughen and Stuart J. Smyth 15.0. Chapter Summary and Objectives 390 15.1. Introduction 390 15.2. History of Genetic Engineering and its Regulation 392 15.3. Regulation of GE Plants 394 15.4. Regulatory Flaws and Invalid Assumptions 402 15.5. The State of Genome Editing Regulation 406 15.6. Conclusion 409 Life Box 14.1. Alan Mchughen 411 References 412 16. Field Testing of Transgenic Plants: Risk Assessment and Performance 415 Detlef Bartsch, Achim Gathmann, Arti Sinha, and Christiane Saeglitz 16.0. Chapter Summary and Objectives 415 16.1. Introduction 416 16.2. Environmental Risk Assessment Process 416 16.3. An Example Risk Assessment: The Case of Bt Maize 418 16.4. Proof of Safety Versus Proof of Hazard 422 16.5. Modeling the Risk Effects on a Greater Scale 422 16.6. Proof of Benefits: Agronomic Performance 423 16.7. Conclusions 424 Life Box 18.1. Detlef Bartsch 426 References 427 17. Intellectual Property in Agricultural Biotechnology: Strategies for Open Access 429 Gregory Graff, David Jefferson, Monica Alandete-Saez, Cecilia Chi-Ham, Sara Boettiger, and Alan B. Bennett 17.0. Chapter Summary and Objectives 429 17.1. Intellectual Property and Agricultural Biotechnology 430 17.2. The Relationship Between Intellectual Property and Agricultural Research 433 17.3. Patenting Plant Biotechnology: The Anti- Commons Problem 434 17.4. What Is Freedom to Operate? 438 17.5. Strategies for Open Access 441 17.6. Conclusions 443 Life Box 17.1. Alan Bennett 444 Life Box 17.2. Maud Hinchee 445 References 446 18. Why Transgenic Plants Are So Controversial 451 Jennifer Trumbo and Douglas Powell 18.0. Chapter Summary and Objectives 451 18.1. Introduction 452 18.2. Perceptions of Risk 454 18.3. Responses of Fear 456 18.4. Feeding Fear: Case Studies 457 18.5. How Many Benefits Are Enough? 459 18.6. Continuing Debates 460 18.7. Business and Control 462 18.8. Conclusions 462 Life Box 18.1. Wayne Parrott 464 References 465 19. Plant Synthetic Biology 467 Scott C. Lenaghan 19.0. Chapter Summary and Objectives 467 19.1. What is Synthetic Biology? 467 19.2. Design: Plant Synthetic Biology 470 19.3. Build: Components of Plant Synthetic Biology 474 19.4. Test: Components of Plant Synthetic Biology 485 19.5. Conclusion 485 Life Box 19.1. Nicola J. Patron 486 Life Box 19.2. Scott C. Lenaghan 487 References 488 Index 491
C. Neal Stewart Jr., PhD, holds the Racheff Chair of Excellence in Plant Molecular Genetics and is a Professor in the Department of Plant Sciences at the University of Tennessee, Knoxville. He also serves as co-director of the Center for Agricultural Synthetic Biology, which Stewart co-founded in 2018. In addition to the prior editions of Plant Biotechnology, he has written Weedy and Invasive Plant Genomics, Plant Transformation Technologies, and Research Ethics for Scientists: A Companion for Students, all published by Wiley.
