Mononuclear Non-heme Iron Dependent Enzymes, Volume 703 PART B focuses on methods for studying, characterizing, and leveraging the chemistry of mononuclear non-heme iron dependent enzymes. Chapters in this new release include Photoreduction for Rieske oxygenase chemistry, Insights into the Mechanisms of Rieske Oxygenases from Studying the Unproductive Activation of Dioxygen, Non-heme iron and 2-oxoglutarate enzymes catalyze cyclopropane and azacyclopropane formations, Obtaining precise metrics of substrate positioning in Fe(II)/2OG dependent enzymes using Hyperfine Sublevel Correlation Spectroscopy, Xe-pressurization studies for revealing substrate-entrance tunnels, and much more.
Additional chapters cover A tale of two dehydrogenases involved in NADH recycling, Rieske oxygenases and/or their partner reductase proteins, Expression, assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays, Biocatalysis and non-heme iron enzymes, In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01, Structure and function of carbazole 1,9a-dioxygenase, Characterization of a Mononuclear Nonheme Iron-dependent Mono-oxygenase OzmD in Oxazinomycin Biosynthesis, and much more.
Volume editor:
Jennifer Bridwell-Rabb
Imprint: Academic Press Inc
Country of Publication: United States
Dimensions:
Height: 229mm,
Width: 152mm,
Weight: 450g
ISBN: 9780443346477
ISBN 10: 044334647X
Series: Methods in Enzymology
Pages: 380
Publication Date: 24 September 2024
Audience:
Professional and scholarly
,
Undergraduate
Format: Hardback
Publisher's Status: Active
1. Structural studies of mononuclear non-heme iron dependent enzymes Yifan Wang 2. Observing extradiol dioxygenases in action through a crystalline lens Ramaswarmy Subramanian 3. Methods used to determine the structure of the oxygenase component of naphthalene 1,2-dioxygenase Pravindra Kumar 4. Expression, purification, kinetics, and crystallization of non-heme mononuclear iron enzymes: biphenyl, phthalate, and terephthalate dioxygenase Adam Offenbacher 5. Substitution of the Mononuclear, Non-heme Iron Cofactor in Lipoxygenases for Structural Studies: Non-heme iron enzyme dependent reactions Wei-Chen Chang 6. Cyclopropanation and aziridination catalyzed by non-heme iron and 2-oxogluatarate dependent enzymes Hung-wen (Ben) Liu 7. Expression, purification, and characterization of non-heme Iron-dependent mono-oxygenase OzmD in Oxazinomycin Biosynthesis Wenjun Zhang 8. Isonitrile Biosynthesis by a Non-Heme Iron(II)-Dependent Oxidases/Decarboxylase Ikuro Abe 9. Functional analysis of an a-ketoglutarate-dependent non-heme iron oxygenase in fungal meroterpenoid biosynthesis Yisong Guo 10. Spectroscopic and Computational Studies of a Bifunctional Iron- and 2-oxoglutarate Dependent Enzyme, AsqJ Shaun McKinnie 11. Expression, purification, and biochemical characterization of micro- and macroalgal kaoinoid synthases John Lipscomb 12. Approaches to determination of the mechanism of the Rieske monooxygenase salicylate 5-hydroxylase Timothy Bugg 13. In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays Christopher Schofield 14. Methods for production and assaying catalysis of isolated recombinant human aspartate/asparagine--hdyroxylase Paul Fitzpatrick 15. Biochemical and Biophysical Approaches to Characterization of the Aromatic Amino Acid Hydroxylases
