Mononuclear Non-heme Iron Dependent Enzymes, Volume 703 focuses on methods for studying, characterizing, and leveraging the chemistry of mononuclear non-heme iron dependent enzymes. Chapters in this new release include Photoreduction for Rieske oxygenase chemistry, Insights into the Mechanisms of Rieske Oxygenases from Studying the Unproductive Activation of Dioxygen, Non-heme iron and 2-oxoglutarate enzymes catalyze cyclopropane and azacyclopropane formations, Obtaining precise metrics of substrate positioning in Fe(II)/2OG dependent enzymes using Hyperfine Sublevel Correlation Spectroscopy, Xe-pressurization studies for revealing substrate-entrance tunnels, and much more.
Additional chapters cover A tale of two dehydrogenases involved in NADH recycling, Rieske oxygenases and/or their partner reductase proteins, Expression, assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays, Biocatalysis and non-heme iron enzymes, In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01, Structure and function of carbazole 1,9a-dioxygenase, Characterization of a Mononuclear Nonheme Iron-dependent Mono-oxygenase OzmD in Oxazinomycin Biosynthesis, and much more.
1. Photoreduction for Rieske oxygenase chemistry Pinghua Liu 2. Insights into the Mechanisms of Rieske Oxygenases from Studying the Unproductive Activation of Dioxygen Thomas B. Hofstetter, Charlotte E. Bopp and Nora M. Bernet 3. Non-heme iron and 2-oxoglutarate enzymes catalyze cyclopropane and azacyclopropane formations Wei-chen Chang 4. Obtaining precise metrics of substrate positioning in Fe(II)/2OG dependent enzymes using Hyperfine Sublevel Correlation Spectroscopy Alexey Silakov 5. Xe-pressurization studies for revealing substrate-entrance tunnels Jennifer D. Bridwell-Rabb 6. A tale of two dehydrogenases involved in NADH recycling Jennifer D. Bridwell-Rabb 7. Rieske oxygenases and/or their partner reductase proteins Pravindra Kumar 8. Expression, assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays Timothy David Bugg 9. Biocatalysis and non-heme iron enzymes Xiongyi Huang 10. In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01 Sandy Schmidt and Niels A.W. de Kok 11. Structure and function of carbazole 1,9a-dioxygenase Hideaki Nojiri 12. Characterization of a Mononuclear Nonheme Iron-dependent Mono-oxygenase OzmD in Oxazinomycin Biosynthesis Hung-wen Liu 13. Spectroscopic and computational studies of a bifunctional iron- and 2-oxoglutarate dependent enzyme, AsqJ Yisong Guo 14. Non-heme iron oxygenases for natural product biosynthesis Ikuro Abe 15. Isonitrile Formation by a Non-Heme Iron(II)-Dependent Oxidase/Decarboxylase Wenjun Zhang 16. Assays for the epidermal growth factor hydroxylase Asph Christopher Joseph Schofield 17. Amino acid hydroxylases Paul Fitzpatrick 18. Extradiol dioxygenases Yifan Wang 19. Title to be determined Ramaswamy Subramanian 20. Experimental and computational approaches to understanding and addressing the stability limitations of Rieske Oxygenases Jennifer DuBois 21. Construction and Testing of Whole-Cell Non-Heme Iron Biocatalysts Ryan Summers 22. Equilibrium dialysis to measure binding affinities of non-heme iron enzymes Ambika Bhagi-Damodaran 23. title to be determined Thomas C. Brunold 24. Approaches to determination of the mechanism of the Rieske monooxygenase salicylate 5-hydroxylase John D. Lipscomb and Melanie Rogers 25. Preparation of reductases for multicomponent oxygenases Lindsay David Eltis and Megan Wolf 26. Expression, purification, and biochemical characterization of micro- and macroalgal kainoid synthases Shaun McKinnie 27. Title to be determined Kabirul Islam 28. Title to be determined Edward I. Solomon 29. title to be detrmined Adam Offenbacher
After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.
