Mitochondrial Translocases Part B

1. TOM-TIM23 supercomplex formation Peter Rehling, Naintara Jain and Ridhima Gomkale 2. Analysis of inner membrane lateral sorting at the preseqeunce translocase Hyun Kim 3. Monitoring alpha-helical membrane protein insertion into the outer mitochondrial membrane of yeast cells Thomas Becker 4. Monitoring alpha-helical membrane protein insertion into the outer mitochondrial membrane of human cells Rebecca Voorhees 5. Mass spectrometry based analysis of mitochondrial protein translocation mutants and interactome studies Bettina Warscheid 6. Analysis of protein trafficking between mitochondria and the endoplasmic reticulum by microscopy Toshiya Endo 7. Monitoring retro-translocation of mitochondrial intermembrane space proteins Piotr Bragoszewski and Magda Krakowczyk 8. Analysis of the evolutionary conservation of mitochondrial biogenesis pathways Jeremy Grant Wideman and Kamawela P. Leka 9. Analysis of protein-protein interaction of the mitochondrial translocase at work by using technically effective BPA photo-crosslinking method Takuya Shiota and Edward Germany 10. Analysis of mitochondrial protein translocation by disulfide bond formation and cysteine specific crosslinking Nils Wiedemann and Laura F. Fielden 11. Analysis of the mitochondrial distribution of mitochondrial translocase complexes Stefan Jacobs 12. ANALYSIS OF MITOCHONDRIAL TRANSLOCASES TOM AND TIM BY THE PATCH-CLAMPING TECHNIQUE Maria Luisa Campo 13. Large-scale purification of mitochondrial protein complexes in yeast expression system for structural analyses Yuhei ????(???), Nanako Kobayashi, Yuta Imai, Shintaro Kita and Shiho Kawai 14. Generation of TIM chaperone substrate complexes Paul Schanda, Undina Guillerm and Iva Sucec 15. Purification of functional recombinant human mitochondrial Hsp60 Abdussalam Azem and Celeste Weiss- Katz 16. Analysis of mitochondrial protein complex assembly by blue native PAGE in knock out cell lines David A. Stroud, David Robinson and Liana Semcesen 17. Analysis of mitochondrial protein aggregation and disaggregation Wolfgang Voos, Anna Wilkening, Robin Ostermann, Michael Bruderek, Witold Jaworek and Laura Ruland 18. Monitoring mitochondrial protein translocation regulation by phosphorylation Chris Meisinger 19. Analysis of mitochondrial biogenesis regulation by oxidative stress Naresh Babu V. Sepuri 20. Methods to analyze the mitochondrial unfolded protein response Cole Haynes 21. Analysis of quality control pathways for the translocase of the outer mitochondrial membrane Fabian den Brave and Lara Calvo Santos 22. Methods to analyze mitochondria associated degradation Liza Pon 23. Monitoring and modulation of mitochondrial redox states Jan Riemer 24. Seahorse assay for the mitochondrial respiration using Saccharomyces cerevisiae as a model system Patrick D'silva 25. Screen for temperature-sensitive mutants of non-essential yeast genes Nils Wiedemann, Nikolaus Pfanner and Iniyan Ganesan