Mitochondrial Translocases Part A

1. Isolation of yeast mitochondria by differential centrifugation Heike Rampelt 2. Isolation of yeast mitochondria by affinity purification using magnetic beads Pin-Chao Liao, Liza Pon, Tzu-Ying Lin and Shih-Hung Chien 3. A modified procedure for the separation of mitochondria from peroxisomes Doron Rapaport and Nitya Aravindan 4. Subcellular fractionation for the analysis of mitochondrial proteins Nils Wiedemann and Conny Steiert 5. Monitoring mitochondrial localization of dual localized proteins using a Bi-Genomic Mitochondrial-Split-GFP Hubert Dominique Becker 6. Analysis of mitochondrial localization and biogenesis by genetic screens and automated imaging Maya Schuldiner and Yury S. Bykov 7. Methods to monitor mitochondrial disulfide bonds Agnieszka Chacinska, Ben Hur Mussulini, Michal Wasilewski and Klaudia K Maruszczak 8. Prediction of mitochondrial targeting signals and their cleavage sites Paul Horton, Kenichiro Imai and Yoshinori Fukasawa 9. Monitoring of mitochondrial preprotein processing, peptide accumulation and degradation Nora Vögtle and Cansu Kücükköse 10. Analysis of mitochondrial targeting signal cleavage and processing by mass spectrometry Pitter F. Huesgen 11. Analysis of targeting signals for mitochondrial intermembrane space import Kostas Tokatlidis 12. Analysis and prediction of internal mitochondrial targeting signals Timo Mühlhaus, Johannes M. Herrmann, Saskia Rödl and Felix Jung 13. Monitoring and analysis of mitochondrial precursor protein aggregates in the cytosol Agnieszka Chacinska 14. In vitro translation of mitochondrial precursor proteins in yeast extract to study interactions with cytosolic chaperones Layla Drwesh 15. In vitro import of mitochondrial precursor proteins into yeast mitochondria Dejana Mokranjac 16. Monitoring the import and assembly of mitochondrial precursor proteins into human cell line mitochondria Diana Stojanovski and Jordan Crameri 17. Import of mitochondrial precursor proteins into mitochondria with semi- intact cells Johannes M. Herrmann 18. The MitoLuc Assay for the Analysis of the Mechanism of Mitochondrial Protein Import Ian Collinson, Hope Isobel Needs, Youmian Yan and Natalie Niemi 19. Monitoring mitochondrial protein import by live cell imaging FX Reymond Sutandy 20. Quantification of mitochondrial protein import by mass spectrometry Christian Münch 21. Methods to analyze mitochondrial protein translocation in plant mitochondria Monika W. Murcha 22. In organello silencing of mitochondrial gene expression Luis Daniel Cruz-Zaragoza and Mats Koschel 23. Assays to monitor mitochondrial translation Martin Ott 24. Analysis of mitochondrial translation using click chemistry Sven Dennerlein and Roya Yousefi