<br> It is indeed an excellent compilation of the basic steps in immunoassay design and development. Dr. Gosling has assembled an accomplished group of immunoassay experts who provide clear and uncomplicated accounts of the practical aspects of immunoassays. A useful feature of the book is the inclusion of easily identified, step-by-step protocols and tips throughout the text. This book will be an invaluable practical resource for students and research workers trying to come to grips with the complexities of immunoassay technology. -- Larry J. Kricka, Clinical Chemistry, 47, No. 3, 2001<p><br> There are now so many variations on the use of the core technology of the immunoassay, the antigen-antibody reaction, that when I opened this book I was a little sceptical as to whether James Gosling would be able to achieve the goals set for the Practical Approach series. ... [A]ny text that sets out to provide a series of protocols to help the experimental scientist to measure a specific analyte must ensure that there is guidance on method design, optimization, calibration and validation--that is, to keep the feet on the ground! The book achieves this objective and includes useful tips and contact names and, although the latter will go out of date, it adds value. ... This is a useful book for the analyst who needs to develop and use an immunoassay. It is also a useful text for the analyst who uses a 'commercial assay' and who has not had any part in its development, because it provides the background knowledge that will help with troubleshooting. --Trends in Biochemical Sciences<p><br>