This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in-situ hybridization, freeze substitution and the utilization of ultra-small probes and replicas for use by viologists and electron microscopists identifying and studying viruses, their components and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.
By:
Alexander D. Hyatt,
Bryan Eaton,
Bryan Eaton (Australian Animal Health Lab.,
Geelong,
Australia)
Contributions by:
Wolfgang Bohn,
Peter Faulkner (Queen's University)
Imprint: CRC Press Inc
Country of Publication: United States
Dimensions:
Height: 234mm,
Width: 156mm,
Spine: 235mm
Weight: 1.020kg
ISBN: 9780849367595
ISBN 10: 084936759X
Pages: 464
Publication Date: 10 November 1992
Audience:
College/higher education
,
Professional and scholarly
,
Professional & Vocational
,
A / AS level
,
Further / Higher Education
Format: Hardback
Publisher's Status: Active
Immuno-Gold Electron Microscopy for Virus Diagnosis: Immune Complexing (E.L. Palmer and M.L. Martin). Solid-Phase Immune Electron Microscopy of Virus Preparations (R.G. Milne). Gold Labeling in Negative Stain Immune Electron Microscopy (P.J.C. Dopping-Hepenstal and J.E. Beesley). The Grid-Cell-Culture Technique (A.D. Hyatt and B.T. Eaton). Use of Microspheres for Immune Scanning Electron Microscopy (J.P. Andersson, U.G. Andersson, and A. Sonnerborg). Immuno-Gold Electron Microscopy in Virus Research: The Colloidal Gold Probe, An Overview of Its Application in Viral Cytochemistry (S. Garzon and M. Bendayan). Detection of Viral and Cellular Structures by Post-Embedding Immunocytochemistry (G.N. Williams and P. Faulkner). Multiple Labeling of Thin Sections (M. Nielsen and L. Bastholm). Immuno-Gold Replica Technique for Study of Virus-Infected Cells (K. Mannweiler, H. Hohenberg, G. Rutter, and W. Bohn). Detection and Analysis of Viral Proteins by Immuno-Gold Labeling (K.G. Murti). Cryo-Sectioning (H.K. Hagler). Improvements of Immuno Dection Using Ultra-Small Gold Probes and Cryosections (J.L.M. Leunissen and P. van de Plas). Freeze Substitution in Virus Research: A Preview (H. Schwartz, H. Hohenberg, and B.M. Humbel). Ultrastructural In Situ Hybridization Using Immuno-Gold (G.L. Bassell and R.H. Singer). Use of Colloidal Gold and Silver Enhancement in Scanning Electron Microscopy (A.D. Hyatt and S.M. Brookes).
Alexander D. Hyatt, Bryan Eaton
