Biophysical Approaches for the Study of Membrane Structure, Part A, Volume 700 explores lipid membrane asymmetry and lateral heterogeneity. A burst of recent research has shown that bilayers whose leaflets differ in their physical properties—such as composition, phase state, or lateral stress—exhibit many fascinating new characteristics, but also pose a host of new challenges related to their creation, characterization, simulation, and theoretical description. Chapters in this new release include Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging FCS, Effects of lateral and hydrostatic pressure on membrane structure and properties, and much more.
Other sections cover Using the yeast vacuole as a system to test the lipid drivers of membrane heterogeneity in living cells, Direct quantification of cellular membrane lipids using ratiometric fluorescence sensors, The spectral phasor approach to resolving membrane order with environmentally sensitive dyes, The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domains, Advanced microscopy methods to study membrane pores, Use of cryo-EM to study membrane phase separation, and much more.
1. Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging FCS Nirmalya Bag, Arpita Tripathy and Sudipti Priyadarsinee 2. Fluorescence imaging of lamellipodin mediated biomolecular condensates on solid supported lipid bilayer membranes Tobias Baumgart 3. Effects of lateral and hydrostatic pressure on membrane structure and properties Nick Brooks 4. Using the yeast vacuole as a system to test the lipid drivers of membrane heterogeneity in living cells Itay Budin, Israel Juarez-Contreras and Hyesoo Kim 5. Direct quantification of cellular membrane lipids using ratiometric fluorescence sensors Wonhwa Cho 6. The spectral phasor approach to resolving membrane order with environmentally sensitive dyes Rumiana Dimova and Agustín Mangiarotti 7. The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domains Thais A. Enoki 8. Advanced microscopy methods to study membrane pores Ana J. García-Sáez 9. Use of cryo-EM to study membrane phase separation Frederick Heberle and M. Neal Waxham 10. Dark secrets of substrate preparation for patterned supported bilayer experiments Aurelia Honerkamp-Smith 11. Using lipid binding proteins to study lipid membrane heterogeneity TOSHIHIDE KOBAYASHI 12. Structural characterization of lateral phase separation in polymer-lipid hybrid membranes Cecília Leal, Nurila Kambar, Minh N. Do, Corey Snyder and Yoo Kyung Go 13. Applications of phase-separating multi-bilayers in protein binding/partitioning/signaling and membrane-associated condensate formation Kandice R. Levental and Hong-Yin Wang 14. Studying lipid flip-flop using NMR and TR-SANS Drew Marquardt, Dominik Dziura and Maksymilian Dziura 15. Measuring bilayer thickness from cryo-TEM of hydroperoxidized POPC and SOPC Carlos Marques and André Schroder 16. Structure of symmetric and asymmetric lipid membranes from joint SAXS/SANS Georg Pabst, Enrico Semeraro and Moritz Frewein 17. Quantification of membrane geometry and protein sorting on cell membrane protrusions using fluorescence microscopy Zheng Shi 18. A TIRF microscopy-based method for observing the uptake of individual particles by clathrin mediated endocytosis Jeanne Stachowiak, Grant Ashby, Kayla Keng and Carl Hayden 19. Pore-spanning membranes as a tool to investigate lateral lipid membrane heterogeneity Claudia Steinem and Larissa Socrier 20. MaCD based plasma membrane outer leaflet lipid exchange in mammalian cells to study insulin receptor activity Pavana Suresh and Erwin London
After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.
