Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.
Volume 2 contains 29 chapters with updated techniques for industrial plants, root plants, nuts and fruits, tropic plants, and other important plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers in both fundamental and applied biology.
Edited by:
Kan Wang
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: 3rd ed. 2015
Volume: 1224
Dimensions:
Height: 254mm,
Width: 178mm,
Spine: 22mm
Weight: 9.746kg
ISBN: 9781493916573
ISBN 10: 1493916572
Series: Methods in Molecular Biology
Pages: 378
Publication Date: 22 November 2014
Audience:
Professional and scholarly
,
Undergraduate
Format: Hardback
Publisher's Status: Active
Brassica Rapa.- Cotton (Gossypium Hirsutum L.).- Jatropha (Jatropha Curcas L.).- Sesame (Sesamum Indicum L.).- Sunflower (Helianthus Annuus L.).- Carrot (Daucus Carota L.).- Cassava (Manihot Esculenta Cranz).- Potato (Solanum Tuberosum L.).- Taro (Colocasia Esculenta (L.) Schott).- Apricot (Prunus Armeniaca L.).- Blueberry (Vaccinium Corymbosum L.).- Cherry.- Chestnut, American (Castanea Dentata (Marsh.) Borkh.).- Chestnut, European (Castanea Sativa).- Grapevine (Vitis Vinifera L.).- Melon (Cucumis Melo).- Peach (Prunus Persica L.).- Strawberry (Fragaria X Ananassa).- Walnut (Juglans).- Citrus Transformation Using Juvenile Tissue Explants.- Citrus Transformation Using Mature Tissue Explants.- Coffee (Coffea Arabica L.).- Pineapple [Ananas Comosus (L.) Merr.].- Sugarcane (Saccharum Spp. Hybrids).- Hemp (Cannabis Sativa L.).- Orchids (Cymbidium Spp., Oncidium And Phalaenopsis).- Poinsettia (Euphorbia Pulcherrima Willd. Ex Klotzsch).- Populus Trichocarpa.- Tall Fescue (Festuca Arundinacea Schreb.).
