This book covers the principle, structure, enhancement of sensitivity and resolution power of photothermal and Raman microscopies. It includes real-world applications to biological and medical targets.
Advanced Microscopy: Photo-Thermal and Induced-Raman Microscopy introduces clear descriptions of various Raman processes such as spontaneous, stimulates, coherent anti-Stokes Raman (CARS), Raman loss and Stokes Raman (gain). It covers pump-probe microscopies using actinic (pump) laser and sensing (probe) laser resulting in improvement due to intrinsic nonlinearity, which provides an advantage in the imaging of nonfluorescent targets. The author also provides solutions to noise and sensitivity problems which are two of the most important concerns in the microscopy applications. Finally, the book also draws direct comparisons of the advantages and drawbacks of a Raman microscopes in comparison with photothermal microscopes.
The book will be useful to researchers and non-specialists in biomedical fields using optics and electronics relevant to (optical) microscopes. It will also be a helpful resource to graduate students in the fields of biology and medical research who are using photothermal microscopes in their research.
By:
Takayoshi Kobayashi
Imprint: CRC Press
Country of Publication: United Kingdom
Dimensions:
Height: 234mm,
Width: 156mm,
Weight: 975g
ISBN: 9781032758855
ISBN 10: 1032758856
Pages: 434
Publication Date: 15 October 2024
Audience:
College/higher education
,
Professional and scholarly
,
Primary
,
Undergraduate
Format: Hardback
Publisher's Status: Active
Chapter 1- Preface & Introduction Chapter 2.1.1- Optimal detection angle in sub-diffraction resolution photothermal microscopy: application for high sensitivity imaging of biological tissues Chapter 2.1.2- Resolution enhanced pump-probe microscope with a spatial filter Chapter 2.1.3- Reduction of distortion in photothermal microscopy and its application to the high- resolution three-dimensional imaging of nonfluorescent tissues Chapter 2.2.1- Numerical study of the subtraction threshold for fluorescence difference microscopy Chapter 2.2.2- Sub-diffraction-limit imaging using mode multiplexing Chapter 2.2.3- Polarization modulation for fluorescence emission difference microscopy Chapter 2.2.4- Numerical study of super-resolved optical microscopy with partly staggered beams Chapter 2.2.5- Resolution enhancement of pump-probe microscopy with an inverse-annular spatial filter Chapter 3.1- Sub-diffraction resolution pump-probe microscopy with shot-noise limited sensitivity using laser diodes Chapter 3.2- Sensitivity enhancement of photothermal microscopy with radially segmented balanced detection Chapter 3.3- Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy Chapter 4.1.1- Numerical calibration of the spatial overlap for subtraction microscopy Chapter 4.1.2- Polarization modulation for fluorescence emission difference microscopy Chapter 4.1.3- Subtraction threshold for an isotropic fluorescence emission difference microscope Chapter 4.2- Noise cancellation with phase-detection technique for pump-probe measurement and application to stimulated Raman imaging Chapter 4.3- Simultaneous dual-wavelength imaging of nonfluorescent tissues with 3D subdiffraction photothermal microscopy Chapter 5.1- Gray-level co-occurrence matrix analysis of several cell types in mouse brain using resolution-enhanced photothermal microscopy Chapter 5.2- Label-free imaging of melanoma with nonlinear photothermal microscopy Chapter 5.3- Noninvasive, label-free, three-dimensional imaging of melanoma with confocal photothermal microscopy: Differentiate malignant melanoma from benign tumor tissue Chapter 5.4- Label-Free Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiation between Malignant and Benign Tissue Chapter 6.1- Development of a multiplex stimulated Raman microscope for spectral imaging through multi-channel lock-in detection Chapter 6.2- Multiplex stimulated Raman imaging with white probe-light from a photonic-crystal fibre and with multi-wavelength balanced detection Chapter 6.3- Theoretical description for nonlinear dynamic light scattering based on stimulated Raman effect (NLDLS-SRS) O Number 662
Takayoshi Kobayashi was born in Niigata Prefecture in 1944. He is a professor emeritus at the University of Tokyo, from which he previously graduated with Bachelor's, Master's, and Doctorate degrees. He joined the Institute of Physical and Chemical Research (Riken), and between 1977 and 1979, he was a temporary member of the Technical Staff at Bell Laboratories. In 1980 he joined the Department of Physics at the University of Tokyo as an associate professor and was promoted to full professor in 1994. In March 2006 he retired from the university and moved to the Department of Applied Physics and Chemistry at the University of Electro-Communications, in Tokyo. He was appointed Chair Professor and the Director of the National Chiao-tung University in 2006, which became the National Yang-Ming Chiao-Tung University in 2021. His research interests include quantum electronics, laser physics, femtosecond spectroscopy, ultrafast nonlinear optics, quantum optics, chemical physics, quantum information science and technology.
