Adipose-Derived Stem Cells

Methods and Protocols

Jeffrey Gimble, Bruce Bunnell, Trivia Frazier , Cecilia Sanchez

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English

Springer-Verlag New York Inc.
14 March 2024

Developmental biology; Cellular biology (cytology)

Series: Methods in Molecular Biology

Summary
Details

Since the publication of the previous editions, there has been increased focus on the use of adipose-derived stromal/stem cells (ASC) and stromal vascular fraction (SVF) cells in three-dimensional hydrogel-based scaffolds for the development of microphysiological systems (MPS) serving as in vitro humanized assays and alternatives to in vivo pre-clinical animal models. This third edition volume discusses of the latest technology and advancements in the field of human-derived ASC and SVF. The chapters in this book are organized into four parts. Part One focuses on human ASC’s isolation, characterization, and differentiation. Part Two describes the isolation and characterization of ASC and SVF from canine, feline, and murine tissues. Part Three looks at hydrogels, scaffolds, and microphysiological systems, and Part Four talks about the new assays and applications using ASC. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Adipose-Derived Stem Cells: Methods and Protocols, Third Edition is a valuable resource for both novice and experts researchers interested in learning more about this important and developing field.

Edited by: Jeffrey Gimble, Bruce Bunnell, Trivia Frazier, Cecilia Sanchez
Imprint: Springer-Verlag New York Inc.
Country of Publication: United States
Edition: 3rd ed. 2024
Volume: 2783
Dimensions: Height: 254mm, Width: 178mm,
ISBN: 9781071637616
ISBN 10: 1071637614
Series: Methods in Molecular Biology
Pages: 393
Publication Date: 14 March 2024
Audience: Professional and scholarly , Undergraduate
Format: Hardback
Publisher's Status: Active