This laboratory reference compiles the essential protocols used in cyanobacterial research. Cyanobacteria is a model system for biofuel production and sequestration of carbon dioxide to tackle global climate change due to increasing levels of greenhouse gases. Topics range from identification and growth parameters to biomolecule estimation and isolation. It also covers genome analysis and bioengineering of cyanobacteria for novel products.
Key Features:
Provides step-by-step procedures and troubleshooting tips Covers identification, growth parameters and pigment estimation methodologies Describes isolation, characterization and purification of microcystin, phycobiliproteins and scytonemin from cyanobacteria Discusses genomics, proteomics and bioengineering of cyanobacteria from novel products Explains sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria
This collection is useful to students and researchers in life sciences. It is also meant for industry experts who are involved in the production of biofuels, biofertilizers, other value-added products, and carbon sequestration using cyanobacteria.
Edited by:
Shailendra Pratap Singh,
Rajeshwar P. Sinha,
Donat-P. Häder
Imprint: CRC Press
Country of Publication: United Kingdom
Dimensions:
Height: 254mm,
Width: 178mm,
Weight: 630g
ISBN: 9781032498348
ISBN 10: 103249834X
Pages: 235
Publication Date: 14 June 2024
Audience:
College/higher education
,
Professional and scholarly
,
Primary
,
Undergraduate
Format: Hardback
Publisher's Status: Forthcoming
Chapter 1: Evaluating the polyphasic approach in cyanobacterial taxonomy Chapter 2: Estimation of growth and photosynthetic pigments in cyanobacteria Chapter 3: Quantitative estimation of total carbohydrate, protein and lipid contents in cyanobacteria Chapter 4: Isolation and purification of phycobiliproteins Chapter 5: Methods for estimation of oxidative stress indices in cyanobacteria Chapter 6: Estimation of glutamine synthetase enzyme Chapter 7: Estimation of nitrate reductase enzyme Chapter 8: Estimation of nitrogenase enzyme activity in cyanobacteria Chapter 9: Isolation and purification of cyanobacterial toxins Chapter 10: Isolation and purification of scytonemin Chapter 11: Isolation and purification of mycosporine-like amino acids Chapter 12: Protein estimation in cyanobacterial samples Chapter 13: Proteomic analysis of cyanobacteria employing 2-dimensional gel electrophoresis (2-DE) Chapter 14: LC-MS/MS based proteomics study of cyanobacteria Chapter 15: Monitoring photosynthetic performance in cyanobacteria by a modulated fluorometer Chapter 16: Reactive oxygen species measurement in cyanobacteria using 2′,7′- dichlorodihydrofluorescein diacetate (DCFH-DA) dye and fluorescence microscope or flow cytometer Chapter 17: Flow cytometry-based methods for estimating DNA content and live-dead cells in cyanobacteria Chapter 18: Genomic DNA and RNA extraction from cyanobacteria Chapter 19: Detection and quantification of DNA damage in cyanobacteria by immunodot- blot assay Chapter 20: Engineering cyanobacteria for the synthesis of novel products Chapter 21: Sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria Chapter 22: Cyanobacterial biofertilizer production technology Chapter 23: Biomass composition measurement and intracellular flux analyses for insights into cyanobacterial metabolism Chapter 24: In silico genome assembly and functional annotation Index
Dr. Shailendra P. Singh is an Assistant Professor at the Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University (BHU), Varanasi, India. He earned his PhD from the Department of Biology, Friedrich-Alexander University, Erlangen-Nuremberg, Germany. Prof. Dr. Rajeshwar P. Sinha, DAAD Fellowship Awardee and Fellow, Society for Applied Biotechnology, India, is a Professor of Molecular Biology, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University (BHU), Varanasi, India. Prof. Donat-P. Häder is the past-Director of the Botanical Institute and held the chair of Ecophysiology of Plants at the Friedrich-Alexander University, Erlangen-Nürnberg, Germany. He was also the director of the Botanical Garden.
